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小鼠抗EYFP Tag单克隆抗体

价格: 在线咨询
货号:
KT011421
产品规格:
100ul
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项
中文名称:  小鼠抗EYFP Tag单克隆抗体

英文名称: Anti-EYFP Tag mouse monoclonal antibody

别       名: Enhanced Yellow florescent protein; eYFP; YFP; Yellow Fluorescent Protein; enhanced Yellow Fluorescent Protein

相关类别: 标签抗体

储       存: 冷冻(-20℃)

宿       主: Mouse

抗       原: EYFP Tag

标  记  物: Unconjugate

克隆类型: mouse monoclonal

技术规格


Background:

Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.

Applications:

WB

Name of antibody:

EYFP Tag

Immunogen:

Fusion protein of EYFP

Full name:

Enhanced Yellow florescent protein

Synonyms:

eYFP; YFP; Yellow Fluorescent Protein; enhanced Yellow Fluorescent Protein

WB Positive control:

Recombinant EYFP Tag protein

WB Recommended dilution:

5000-20000

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