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产品详情
操作说明
注意事项
Product Information
| Description |
EIF2S1 mouse monoclonal |
| Protein full name |
Eukaryotic translation initiation factor 2 subunit 1 |
|
Synonyms |
EIF2S1, EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, eukaryotic translation initiation factor 2 subunit alpha |
| Immunogen |
Recombinant protein corresponding to Mouse EIF2S1 |
| Isotype |
IgG |
| Purity |
Affinity purification |
|
Predicted MW. |
36 kDa |
|
Observed MW. |
38 kDa |
| Subcellular location |
Cytoplasmic granule |
| Uniprot ID |
Applications
| Applications |
WB |
IHC |
IF |
| Species |
Human, Mouse, Rat |
Mouse, Rat |
Mouse, Rat |
| Dilution |
1: 500-1: 1000 |
1: 4000-1: 8000 |
1: 2000-1: 4000 |
| Positive tissue |
colon, brain |
brain |
brain |
Background
Eukaryotic translation initiation factor 2 subunit 1 (eIF2α) is a protein that in humans is encoded by the EIF2S1 gene. The protein encoded by this gene is the alpha (α) subunit of the translation initiation factor eIF2 complex which catalyzes an early regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA (Met-tRNAiMet) to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (α, 36 kD, this article), beta (β, 38 kD), and gamma (γ, 52 kD). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2α.
Images
|
Western blot analysis of EIF2S1 (GB12544) at dilution of 1: 1000. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: A549 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC12 cell lysate Lane 6: Mouse colon tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat colon tissue lysate Lane 9: Rat brain tissue lysate |
|
Immunohistochemistry analysis of paraffin-embedded mouse brain using EIF2S1 (GB12544) at dilution of 1: 6000 |
|
Immunohistochemistry analysis of paraffin-embedded rat brain using EIF2S1 (GB12544) at dilution of 1: 6000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
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地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室