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Anti -Vimentin Mouse mAb

价格: 在线咨询
货号:
KT011582
产品规格:
100 μL
Applications:
WB | IHC | IF
Species:
H,M,R | M,R
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

Vimentin mouse monoclonal antibody

Protein full name

Vimentin

Synonyms

VIM, CTRCT30, HEL113, vimentin

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse vimentin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

57 kDa

Observed MW.

57 kDa

Uniprot ID

P08670, P20152, P31000




Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 1000-1: 2000

lung

IHC/IF

Mouse, Rat

1: 500-1: 1000

colon, thymus, tonsil



Background


Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.




Images



Picture 1.png

Western blot analysis of Vimentin (GB12192) at dilution of 1: 1000

Picture 2.png

Immunohistochemistry of paraffin embedded human tonsil using vimentin (GB12192) at dilution of 1: 1000 (400x lens)

Picture 3.png

Immunofluorescence of paraffin embedded mouse colon using vimentin (GB12192) at dilution of 1: 500 (400x lens)

Picture 4.png

Immunofluorescence of paraffin embedded mouse thymus using vimentin (GB12192) at dilution of 1: 500 (400x lens)




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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