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Anti -Tyrosine Hydroxylase Rabbit pAb

价格: 在线咨询
货号:
KT011648
产品规格:
100 μL
Applications:
WB/IHC/IF
Species:
M/R,H/M/R,H/M/R
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

Tyrosine Hydroxylase rabbit polyclonal antibody

Protein full name

Tyrosine 3-monooxygenase

Synonyms

TH, Th, DYT14, DYT5b, TYH, tyrosine hydroxylase, Tyrosine hydroxylase

Immunogen

Recombinant protein corresponding to Mouse Tyrosine Hydroxylase

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Axon

Predicted MW.

62 kDa

Observed MW.

62 kDa

Uniprot ID

P24529, P04177



Applications


Applications

Species

Dilution

Positive Sample

WB

Mouse, Rat

1: 500-1: 1000

U-251MG, C6, Neuro-2a

IHC/IF

Human, Mouse, Rat

1: 500-1: 2000

corpus striatum, substantia nigra



Background


Tyrosine hydroxylase or tyrosine 3-monooxygenase is the enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA). It does so using molecular oxygen(O2), as well as iron (Fe2+) and tetrahydrobiopterin as cofactors. L-DOPA is a precursor for dopamine, which, in turn, is a precursor for the important neurotransmitters norepinephrine (noradrenaline) and epinephrine(adrenaline). Tyrosine hydroxylase catalyzes the rate limiting step in this synthesis of catecholamines.




Images




Western blot analysis of Tyrosine Hydroxylase (GB11181) at dilution of 1: 500

Immunohistochemistry of paraffin embedded mouse corpus striatum using Tyrosine Hydroxylase (GB11181) at dilution of 1: 1000 (200x lens)

Immunohistochemistry of paraffin embedded mouse substantia nigra using Tyrosine Hydroxylase (GB11181) at dilution of 1: 1000 (400x lens)

Immunohistochemistry of paraffin embedded rat corpus striatum using Tyrosine Hydroxylase (GB11181) at dilution of 1: 1000 (200x lens)

Immunohistochemistry of paraffin embedded rat substantia nigra using Tyrosine Hydroxylase (GB11181) at dilution of 1: 1000 (200x lens)

Picture 6.png

Immunofluorescence of paraffin embedded mouse corpus striatum using TH (GB11181) at dilution of 1: 500 (400x lens)

Picture 7.png

Immunofluorescence of paraffin embedded mouse substantia nigra using TH (GB11181) at dilution of 1: 500 (400x lens)

Picture 8.png


Immunofluorescence of paraffin embedded rat corpus striatum using TH (GB11181) at dilution of 1: 500 (400x lens) 




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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