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Anti -PCNA Rabbit pAb

价格: 在线咨询
货号:
KT011682
产品规格:
Applications:
WB | IHC | IF
Species:
H/HM
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

PCNA rabbit polyclonal antibody

Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antige

Immunogen

Recombinant protein corresponding to Mouse PCNA

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW.

29 kDa

Observed MW.

36 kDa

Uniprot ID

Q12004, P17918, P04961


Applications


Applications

Species

Dilution

Positive Sample

WB

Human

1: 500-1: 2000

testis, colon

IHC/IF

Human, Mouse

1: 400-1: 2000

lung cancer, ovarian cancer, testis


Background



Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.




Images



Picture 1.png

Western blot analysis of PCNA (GB11010-1) at dilution of 1: 500

Picture 2.png

Immunofluorescence of paraffin embedded human breast cancer using PCNA (GB11010-1) at dilution of 1: 400 (300x lens)

Picture 3.png

Immunofluorescence of paraffin embedded human breast cancer using PCNA (GB11010-1) at dilution of 1: 400 (400x lens)

Picture 4.png

Immunofluorescence of paraffin embedded mouse testis using PCNA (GB11010-1) at dilution of 1: 400 (250x lens)




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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