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Anti -MCM2 Rabbit pAb
价格：
在线咨询
货号：
KT012112
产品规格：
100 μL
Applications：
WB/IHC/IF
Species：
HMR/MR/MR
购买数量：
在线咨询
400-1016-218
产品详情
操作说明
注意事项
Product Information
|
Description |
MCM2 rabbit polyclonal |
|
Protein full name |
DNA replication licensing factor MCM2 |
|
Synonyms |
Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, Bm28, Cdcl1, Kiaa0030, Mcmd2 |
|
Immunogen |
Recombinant protein corresponding to Mouse MCM2 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Nucleus |
|
Predicted MW. |
102 kDa |
|
Observed MW. |
130 kDa |
|
Uniprot ID |
P49736, P97310, D3ZP96 |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
lymph node, thymus |
|
IHC |
Mouse, Rat |
1: 500-1: 2000 |
spleen, small intestine, colon |
| IF |
Mouse, Rat |
1: 1800-1: 3600 |
|
Background
The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.
Images
|
|
Western blot analysis of MCM2 (GB111361) at dilution of 1: 1000 Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: K562 cell lysate Lane 5: NIH3T3 cell lysate Lane 6: Mouse lymph node tissue lysate Lane 7: Mouse thymus tissue lysate Lane 8: Rat lymph node tissue lysate Lane 9: Rat thymus tissue lysate |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse spleen using MCM2 (GB111361) at dilution of 1: 950 |
|
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Immunohistochemistry analysis of paraffin-embedded rat spleen using MCM2 (GB111361) at dilution of 1: 950 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse small intestine using MCM2 (GB111361) at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded rat colon using MCM2 (GB111361) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室