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Anti -CD3 Rabbit pAb

价格: 在线咨询
货号:
DRF10154
产品规格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,H/M/R,H/M/R
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

CD3 rabbit polyclonal

Protein full name

T-cell surface glycoprotein CD3 epsilon chain

Synonyms

CD3E, IMD18, T3E, TCRE, CD3e molecule

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse CD3

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cell membrane

Uniprot ID

P07766, P22646




Applications


applications

species

dilution

Positive tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

spleen, lung, thymus, 

IHC

Human, Mouse, Rat

1: 250-1: 1000

tonsil, spleen

IF

Human, Mouse, Rat

1: 250-1: 1000

tonsil, spleen



Background


The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.




Images



blob.png

Western blot analysis of CD3 (GB11014) at dilution of 1:1000.

Lane 1:SH-SY5Y cell lysate

Lane 2:THP-1 cell lysate

Lane 3:Raw264.7 cell lysate

Lane 4:Mouse spleen tissue lysate

Lane 5:Mouse lung tissue lysate

Lane 6: Mouse thymus tissue lysate

Lane 7:Rat spleen tissue lysate

Lane 8:Rat lung tissue lysate

Lane 9:Rat thymus tissue lysate


blob.png

Immunohistochemistry analysis of paraffin-embedded human tonsil using CD3 (GB11014) at dilution of 1:500

(Note: pH6.0 citrate buffer high temperature -pressure repairing)


blob.png

Immunohistochemistry analysis of paraffin-embedded mouse Inflammatory spleen using CD3 (GB11014) at dilution of

1:500 (Note: pH6.0 citrate buffer high temperature -pressure repairing)


blob.png

Immunohistochemistry analysis of paraffin-embedded rat spleen using CD3 (GB11014) at dilution of 1:500

(Note: pH6.0 citrate buffer high temperature -pressure repairing)


blob.png

Immunohistochemistry analysis of paraffin-embedded rat Inflammatory spleen using CD3 (GB11014) at dilution of 1:250

(Note: pH6.0 citrate buffer high temperature -pressure repairing)





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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