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Anti -RB-1 Rabbit pAb
价格：
在线咨询
货号：
DRF101235
产品规格：
100 μL
Applications：
IHC/IF
Species：
M/R,M/R
购买数量：
在线咨询
400-1016-218
产品详情
操作说明
注意事项
Product Information
|
Description |
RB-1 rabbit polyclonal |
|
Protein full name |
Retinoblastoma-associated protein |
|
Synonyms |
pRb, Rb, pp105, Rb1 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse RB-1 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
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Uniprot ID |
Applications
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Applications |
Species |
Dilution |
Positive Tissue |
|
IHC |
Mouse, Rat |
1: 700-1: 1400 |
brain, kidney, lung, skeletal muscle |
|
IF |
Mouse, Rat |
1: 500-1: 2500 |
brain, kidney, lung, skeletal muscle |
Background
RB1 belongs to the retinoblastoma protein family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1/H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by Smarxa4/Brg1 by recruiting a histone deacetylase complex to the c-FOS promoter. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Images
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Immunohistochemistry analysis of paraffin-embedded mouse brain using RB-1 (GB111111) at dilution of 1: 1400 |
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Immunohistochemistry analysis of paraffin-embedded rat brain using RB-1 (GB111111) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat kidney using RB-1 (GB111111) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat lung using RB-1 (GB111111) at dilution of 1: 1400 |
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Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using RB-1 (GB111111) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded mouse brain using RB-1 (GB111111) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded rat brain using RB-1 (GB111111) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded rat kidney using RB-1 (GB111111) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded rat lung using RB-1 (GB111111) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded mouse skeletal muscle using RB-1 (GB111111) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室