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Anti -PHD2 / prolyl hydroxylase Rabbit pAb

价格: 在线咨询
货号:
DRF101147
产品规格:
100 μL
Applications:
IHC、IF
Species:
M,R | M,R
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

PHD2 rabbit polyclonal

Protein full name

Egl nine homolog 1

Synonyms

Hypoxia-inducible factor prolyl hydroxylase 2, HIF-PH2, HIF-prolyl hydroxylase 2, HPH-2, Prolyl hydroxylase domain-containing protein 2, PHD2, SM-20, Egln1

Immunogen

Recombinant protein corresponding to Mouse PHD2

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm. Nucleus

Uniprot ID

Q91YE3, P59722




Applications


Applications

Species

Dilution

Positive Tissue

IHC

Mouse, Rat

1: 850-1: 2000

brain, heart

IF

Mouse, Rat

1: 500-1: 1000

brain, heart




Background


Prolyl hydroxylase 2(PHD2) is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402), Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the von Hippel-Lindau ubiquitylation complex.




Images


image.png

Immunohistochemistry analysis of paraffin-embedded mouse brain using PHD2 (GB111030) at dilution of 1: 1700

image.png

Immunohistochemistry analysis of paraffin-embedded mouse heart using PHD2 (GB111030) at dilution of 1: 1700

image.png

Immunohistochemistry analysis of paraffin-embedded rat brain using PHD2 (GB111030) at dilution of 1: 1700




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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