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Anti -MAPRE1/EB1 Rabbit pAb

价格: 在线咨询
货号:
DRF101190
产品规格:
100 μL
Applications:
WB
Species:
H/M
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

EB1 rabbit polyclonal

Protein full name

Microtubule-associated protein RP/EB family member 1

Synonyms

APC-binding protein EB1, End-binding protein 1, EB1, Mapre1

Immunogen

Recombinant protein corresponding to

Mouse EB1

Isotype

IgG

Purity

Affinity purification

Predicted MW.

30 kDa

Observed MW.

35 kDa

Uniprot ID

Q61166




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse

1: 500-1: 1000

colon, thymus




Background


The EB1 protein Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (By similarity). May play a role in cell migration.




Images


Western blot analysis of EB1 (GB111069) at dilution of 1: 1000
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: Mouse colon tissue lysate
Lane 4: Mouse thymus tissue lysate




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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