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Anti -Lck Rabbit pAb

价格: 在线咨询
货号:
DRF101213
产品规格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,M/R,M/R
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

Lck rabbit polyclonal

Protein full name

Proto-oncogene tyrosine-protein kinase LCK

Synonyms

Leukocyte C-terminal Src kinase,
LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Lck, Lsk-t

Immunogen

Recombinant protein corresponding to

Mouse Lck

Isotype

IgG

Purity

Affinity purification

Predicted MW.

58 kDa

Observed MW.

58 kDa

Subcellular location

Cell membrane, Cytoplasm

Uniprot ID

P06239, P06240, Q01621




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

thymus, lymph nodes, spleen

IHC

Mouse, Rat

1: 1500-1: 4000

lung, lymph, spleen, thymus

IF

Mouse, Rat

1: 1500-1: 3000

lung, lymph, spleen




Background


LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.




Images


Western blot analysis of Lck (GB111091) at dilution of 1: 1000

Lane 1: Jurkat cell lysate
Lane 2: Mouse thymus tissue lysate
Lane 3: Mouse lymph nodes tissue lysate
Lane 4: Mouse spleen tissue lysate
Lane 5: Rat thymus tissue lysate
Lane 6: Rat lymph nodes tissue lysate
Lane 7: Rat spleen tissue lysate

Immunohistochemistry analysis of paraffin-embedded mouse lung using Lck (GB111091) at dilution of 1: 1900

Immunohistochemistry analysis of paraffin-embedded mouse lymph using Lck (GB111091) at dilution of 1: 1900

Immunohistochemistry analysis of paraffin-embedded rat lymph using Lck (GB111091) at dilution of 1: 1900

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Lck (GB111091) at dilution of 1: 1900

Immunohistochemistry analysis of paraffin-embedded rat spleen using Lck (GB111091) at dilution of 1: 1900

Immunohistochemistry analysis of paraffin-embedded mouse thymus using Lck (GB111091) at dilution of 1: 1900

Immunohistochemistry analysis of paraffin-embedded rat thymus using Lck (GB111091) at dilution of 1: 1900

Immunofluorescent analysis of paraffin-embedded mouse lung using Lck (GB111091) at dilution of 1: 1900

Immunofluorescent analysis of paraffin-embedded rat lung using Lck (GB111091) at dilution of 1: 1900

Immunofluorescent analysis of paraffin-embedded mouse lymph using Lck (GB111091) at dilution of 1: 1900

Immunofluorescent analysis of paraffin-embedded mouse spleen using Lck (GB111091) at dilution of 1: 3000

Immunofluorescent analysis of paraffin-embedded rat spleen using Lck (GB111091) at dilution of 1: 3000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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