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产品详情
操作说明
注意事项
Product Information
|
Description |
LAT rabbit polyclonal |
|
Protein full name |
Linker for activation of T-cells family member 1 |
|
Synonyms |
36 kDa phospho-tyrosine adapter protein, |
|
Immunogen |
Recombinant protein corresponding to Mouse LAT |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
|
|
Predicted MW. |
26 kDa |
|
Observed MW. |
38 kDa |
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Mouse, Rat |
1: 500-1: 1000 |
spleen, thymus, lymph |
|
IHC |
Human, Mouse, Rat |
1: 1000-1: 4000 |
thymus |
|
IF |
Human, Mouse, Rat |
1: 500-1: 3000 |
thymus |
Background
Linker for activation of T-cells family member 1 (LAT) is a single-pass type III membrane protein which is required for TCR (T-cell antigen receptor) and pre-TCR-mediated signaling, both in mature T-cells and during their development This protein is involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Functionally T cell receptor (TCR) activation results in tyrosine phosphorylation of LAT, leading to the direct interaction with several proteins, including PLC-gamma 1, Grb2 and Gads. These direct ligands then mediate the indirect interaction of LAT with proteins such as SLP-76, Vav1 and Itk. PLC-gamma 1, Vav1 and Itk contain pleckstrin homology (PH) domains that interact with the enzymatic product of phosphoinositide-3-kinase (PI3k).
Images
|
|
Western blot analysis of LAT (GB111123) at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse thymus using LAT (GB111123) at dilution of 1: 2000 |
|
|
Immunohistochemistry analysis of paraffin-embedded rat thymus using LAT (GB111123) at dilution of 1: 2000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室