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Anti -Annexin-4/ANXA4 Rabbit pAb

价格: 在线咨询
货号:
DRF101272
产品规格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,M/R,M/R
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

Annexin-4 rabbit polyclonal

Protein full name

Annexin A4

Synonyms

Annexin IV, Annexin-4, Anxa4, Anx4

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse Annexin-4

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Secreted, Cell Membrane

Predicted MW.

36 kDa

Observed MW.

36 kDa

Uniprot ID

P09525, P97429, P55260




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

heart, liver

IHC

Mouse, Rat

1: 1500-1: 3000

small intestine

IF

Mouse, Rat

1: 1500-1: 3000

small intestine, colon




Background


The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin A4 belongs to the annexin family of calcium dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane related events along exocytotic and endocytotic pathways.  Isolated from human placenta, ANXA4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANXA4 is almost exclusively expressed in epithelial cells.




Images


Western blot analysis of Annexin-4 (GB111147) at dilution of 1: 1000
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: 293T cell lysate
Lane 5: Raw264.7 cell lysate
Lane 6: Mouse heart tissue lysate
Lane 7: Mouse liver tissue lysate
Lane 8: Rat heart tissue lysate
Lane 9: Rat liver tissue lysate

Immunohistochemistry analysis of paraffin-embedded mouse small intestine using Annexin-4 (GB111147) at dilution of 1: 1800

Immunohistochemistry analysis of paraffin-embedded rat small intestine using
Annexin-4 (GB111147) at dilution of 1: 1800

Immunofluorescent analysis of paraffin-embedded mouse small intestine using Annexin-4 (GB111147) at dilution of 1: 1800

Immunofluorescent analysis of paraffin-embedded rat small intestine using Annexin-4 (GB111147) at dilution of 1: 1800

Immunofluorescent analysis of paraffin-embedded rat colon using Annexin-4 (GB111147) at dilution of 1: 1800




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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