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产品详情
操作说明
注意事项
Product Information
|
Description |
TNF-α rabbit polyclonal |
|
Protein full name |
Tumor necrosis factor alpha |
|
Synonyms |
TNF,DIF,TNF-alpha,TNFA,TNFSF2,Tumour necrosis factor, Tumour necrosis factor alpha, TNF-α, tumor necrosis factor, TNLG1 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse TNF-α |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cell membrane, Secreted |
|
Predicted MW. |
26 kDa |
|
Observed MW. |
26 kDa |
|
Uniprot ID |
P01375, P06804 |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 200-1: 500 |
lymph node, thymus, spleen, lung |
|
IHC |
Mouse |
1: 400-1: 1000 |
lung, septic lung, hepatitis |
|
IF |
Mouse |
1: 300-1: 800 |
lung |
Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Images
|
|
Western blot analysis of TNF-α (GB11188) at dilution of 1: 500 |
|
|
Western blot analysis of TNF-α (GB11188) at dilution of 1: 500 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse lung using TNF-α(GB11188)at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse septic lung using TNF-α(GB11188)at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse hepatitis using TNF-α(GB11188)at dilution of 1: 1000 |
|
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse lung using TNF-α(GB11188)at dilution of 1: 400 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
Publications:
|
|
Title |
Journal |
Pubmed ID |
Application |
|
1 |
Early Enteral Nutrition Preserves Intestinal Barrier Function through Reducing the Formation of Neutrophil Extracellular Traps (NETs) in Critically Ill Surgical Patients. |
Oxidative Medicine and Cellular Longevity |
33294125 |
IHC |
|
2 |
QKI deficiency leads to osteoporosis by promoting RANKL-induced osteoclastogenesis and disrupting bone metabolism. |
Cell Death & Disease |
32382069 |
IHC |
|
3 |
Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases. |
Nature Communications |
31040273 |
WB |
|
4 |
Induction of pathological changes and impaired expression of cytokines in developing female rat spleen after chronic excess fluoride exposure. |
Toxicology and Industrial Health |
30463494 |
IHC |
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合肥万物生物科技有限公司
客服热线：400-1016-218
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地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室