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Anti -IL-2 Receptor alpha Rabbit pAb

价格: 在线咨询
货号:
DRF101696
产品规格:
100 μL
Applications:
Species:
购买数量:
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产品详情 操作说明 注意事项

Product Information


Description

IL-2 Receptor alpha rabbit polyclonal

Protein full name

Interleukin-2 receptor subunit alpha

Synonyms

IL2RA, CD25, IDDM10, IL2R, TCGFR, p55, IMD41, interleukin 2 receptor subunit alpha

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse IL2RA

Isotype

IgG

Purity

Affinity purification

Subcellular location

Membrane

Predicted MW.

31 kDa

Observed MW.

45 kDa

Uniprot ID

P01590, P26897




Applications


Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1: 500-1: 1000

splenitis, spleen,

lymph gland, thymus

IHC

Mouse, Rat

1: 200-1: 1000

pneumonia, spleen

IF

Mouse, Rat

1: 250-1: 500

pneumonia, spleen




Background


IL-2Rα(Activated T cells IL-2Rα Chain 55), also kown as membrane interleukin-2 receptor (mIL-2R) or CD25, is a protein encoded by IL2RA gene in Human. This protein is 55 kDa which is the high affinity composition of IL-2R. Few IL-2α chains will express in inactive T cells, while the expression will remarkably increase followed by IL-2 accumulation, when T cell surface receptor (TCR) is active. IL-2αchains will become soluble interleukin-2 receptor (sIL-2R) when they are detached from cell membrane into serum, and the molecular weight of sIL-2R is 45 kDa. The concentration of sIL-2R in serum can be used for a mark to measure T cells activation and IL-2Rα chains expression.




Images



2.jpg

Western blot analysis of IL2RA

(GB11612) at dilution of 1: 1000

Lane 1: Jurkat cell lysate

Lane 2: Mouse splenitis tissue lysate

Lane 3:Mouse spleen tissue lysate

Lane 4: Mouse lymph gland tissue lysate

Lane 5: Mouse thymus tissue lysate

Lane 6: Rat splenitis tissue lysate

Lane 7: Rat spleen tissue lysate

Lane 8: Rat lymph gland tissue lysate

Lane 9: Rat thymus tissue lysate


image.png

Immunohistochemistry analysis of paraffin-embedded mouse pneumonia using

IL2RA (GB11612) at dilution of 1: 500


image.png

Immunohistochemistry analysis of paraffin-embedded mouse spleen using

IL2RA (GB11612) at dilution of 1: 500





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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