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产品详情
操作说明
注意事项
Product Information
|
Description |
CD34 rabbit polyclonal |
|
Protein full name |
Hematopoietic progenitor cell antigen CD34 |
|
Synonyms |
CD34, HPCA1, Mucosialin, Cluster designation 34, cluster of differentiation 34, CD34 antigen |
|
Immunogen |
Recombinant protein corresponding to Human CD34 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Membrane |
|
Predicted MW. |
41 kDa |
|
Observed MW. |
120 kDa |
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
brain, liver |
|
IHC |
Human |
1: 100-1: 400 |
ovarian cancer, esophageal , uterine cancer |
|
IF |
Human |
1: 600-1: 1200 |
colon, kidney, placenta |
Background
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources.
Images
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Western blot analysis of CD34 (GB111693) at dilution of 1: 1000 |
|
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Western blot analysis of CD34 (GB111693) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded human ovarian cancer using CD34 (GB111693) at dilution of 1: 400 |
|
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Immunohistochemistry analysis of paraffin-embedded human esophageal using CD34 (GB111693) at dilution of 1: 400 |
|
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Immunohistochemistry analysis of paraffin-embedded human uterine cancer using CD34 (GB111693) at dilution of 1: 400 |
|
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Immunofluorescent analysis of paraformaldehyde-fixed human colon using CD34 (GB111693) at dilution of 1: 600 |
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Immunofluorescent analysis of paraformaldehyde-fixed human placenta using CD34 (GB111693) at dilution of 1: 600 |
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Immunofluorescent analysis of paraformaldehyde-fixed human kidney using CD34 (GB111693) at dilution of 1: 600 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室