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产品详情
操作说明
注意事项
Product Information
|
Description |
CD20 rabbit polyclonal |
|
Protein full name |
B-lymphocyte antigen CD20 |
|
Synonyms |
MS4A1, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, membrane spanning 4-domains A1 |
|
Immunogen |
Recombinant protein corresponding to Mouse CD20 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
32 KDa |
|
Observed MW. |
37 KDa |
|
Subcellular location |
Membrane |
|
Uniprot ID |
Applications
|
applications |
species |
dilution |
Positive tissue |
|
WB |
Mouse, Rat |
1: 500-1: 1000 |
spleen, lymph gland, thymus |
|
IHC |
Mouse, Rat |
1: 500-1: 3000 |
spleen |
|
IF |
Mouse, Rat |
1: 500-1: 1000 |
lymph, Inflammatory spleen, spleen |
Background
B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. The protein has no known natural ligand[8] and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. It has been shown that CD20 plays a role in the microenvironmental interactions of B cells.
Images
|
Western blot analysis of CD20 (GB11540) at dilution of 1:1000. Lane 1:A20 cell lysate Lane 2:Mouse inflammatory spleen tissue lysate Lane 3:Mouse spleen tissue lysate Lane 4:Mouse lymph gland tissue lysate Lane 5:Rat inflammatory spleen tissue lysate Lane 6:Rat spleen tissue lysate Lane 7:Rat lymph gland tissue lysate Lane 8:Rat thymus tissue lysate |
|
Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD20 (GB11540) at dilution of 1:500 |
|
Immunohistochemistry analysis of paraffin-embedded rat spleen using CD20 (GB11540) at dilution of 1:1000 |
|
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse lymph using CD20 (GB11540) at dilution of 1:1000 |
|
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse Inflammatory spleen using CD20 (GB11540) at dilution of 1:1000 |
|
|
Immunofluorescent analysis of paraformaldehyde-fixed rat spleen using CD20 (GB11540) at dilution of 1:1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室